R-iPS Cell FAQ 2:
What is the expected yield from the in vitro trancription (IVT) reactions?
What is the expected yield from the in vitro trancription (IVT) reactions?
Performed as described, you should recover around 40 ug RNA from each 40 uL IVT reaction.
R-iPS Cell FAQ 3:
How can the success of the RNA synthesis protocol be assessed?
Run
500 ng (5 uL) of the concentration-adjusted products on an E-gel to
check for consistent product yield and relative product sizes, and to
confirm the absence of secondary bands or smears.How can the success of the RNA synthesis protocol be assessed?
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