R-iPS Cell FAQ 1:
What phenotypic changes can be observed during a successful reprogramming trial?
What phenotypic changes can be observed during a successful reprogramming trial?
About
one week out, target fibroblasts should show an involution of
fibroblastic processes, and foci or clusters of epitheliod cells—ideally
with small nuclei, minimal cytoplasm, and signs of ongoing
mitosis—should appear. Colonies with hESC morphology typically start
emerging in ~10-14 days.
From AlleleBlog: http://blog.allelebiotech.com/2012/04/picture-blog-human-mrna-induced-pluripotent-stem-cells-generated-in-days/
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